Detection Instrument: Microplate reader
Test Principal: ALT catalyze the amino conversion reaction between alanine and ?-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37?. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
