Detection Instrument: Microplate reader
Test Principal: The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. ?-amylase was inactivated by the property of amylase not to be heat-resistant, and then the enzyme activity of total amylase and ?-amylase is determined. So the activity of ?-amylase can be calculated indirectly.
