Detection Instrument: Microplate reader
Test Principal: Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.
