IGHM Immunoglobulin Heavy Constant Mu Ig mu chain C regionELK Biotechnology Zebrafish IgM(Immunoglobulin M) ELISA Kit

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Product Name ELK Biotechnology Zebrafish IgM(Immunoglobulin M) ELISA Kit
Catalog No. ELK0801
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Zebrafish Immunoglobulin M concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.21 ?g/mL

Standard: 50 ?g/mL

Detection Range: 0.78-50 ?g/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Infection immunity;Immune molecule;Hematology;

Alternate Names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish IgM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Zebrafish IgM in the samples is then determined by comparing the OD of the samples to the standard curve.