Detection Instrument: Microplate reader
Test Principal: Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.
