Western Blot Substrates

Western Blot is laboratory technique to spate and analyzes any specific protein in the sample containing mixture of different proteins. The technique is used in Cell and Molecular Biology analysis of proteins. The technique has  The overview of the western blot technique include four main steps which include 1) Processing of protein samples for analysis, 2) Separation of size specific proteins by electrophoreses, 3) Transfer of proteins bands on nitrocellulose membrane,  4) determining the proteins with the help of protein specific primary antibodies and secondary antibodies.