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Annexin V Apoptosis Detection Kits

Apoptosis occurs like a signaling cascade of multiple events, providing many opportunities to evaluate the process. During the early stage of apoptosis, the loss of cell membrane asymmetry will take place in which embedded phosphatidylserine (PS) residues in the plasma membrane become externalized. Annexin V is a Ca2+ dependent phospholipid-binding protein that has a high affinity for PS. Flow assays based on Annexin V conjugates accordingly become sensitive quantitative detection of early apoptosis.

Annexin V Apoptosis Detection Kits
Catalogu No. Assay kit and its description                                                                                  

E-CK-A211

Annexin V-FITC/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: FITC
Ex/Em (nm): 490/530
Channel set: FITC
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for Lab Research Use Only.

E-CK-A213

Annexin V-Elab Fluor® 647/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: Elab Fluor® 647
Ex/Em (nm): 650/670
Channel set: APC
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for Lab Research Use Only.

E-CK-A217

Annexin V-APC/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: APC
Ex/Em (nm): 650/660
Channel set: APC
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for lab research use only.

E-CK-A219

Annexin V-EGFP/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: EGFP
Ex/Em (nm): 488/510
Channel set: FITC
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for lab research use only.

E-CK-A221

Annexin V-Cyanine5/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: Cyanine5
Ex/Em (nm): 650/670
Channel set: APC
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for lab research use only.

E-CK-A229

Annexin V-APC/Cyanine7/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: APC/Cyanine7
Ex/Em (nm): 650;760/780
Channel set: APC/Cy7
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for lab research use only.

E-CK-A233

Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: Elab Fluor® Violet 450
Ex/Em (nm): 410/450
Channel set: Pacific Blue
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for lab research use only.

E-CK-A237

Annexin V-Elab Fluor® 488/PI Apoptosis Kit

Detection principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

General Information

Application: Annexin V
Detection method: Fluorometric Method
Sample type: Cell samples
Assay time: 40min
Detection instrument: Flow Cytometer
Dye Type: Elab Fluor® 488
Ex/Em (nm): 495/520
Channel set: FITC
Other reagents required: PBS(E-BC-R187), Deionized water
Storage: 2~8°C, shading light
Shipping: Ice bag
Expiration date: 12 months

The kit is for lab research use only.

 

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